The IDKRAS-3X kit is a ready-to-use multiplex assay for the detection and quantification by digital PCR of mutations in exon 2 (variants G12X and G13X), exon 3 (variants Q61X) and exon 4 (variants A146X and K117).
This optimized reaction system offers a multiplexed solution enabling simultaneous amplification of each sample in a single PCR reaction .
The kit can be used on circulating DNA samples (cf-DNA) extracted from plasma, or on genomic DNA samples (gDNA) isolated from formalin-fixed, paraffin-embedded (FFPE) tissue.
Sample
Circulating free DNA (cf-DNA) extracted from plasma or genomic DNA (gDNA) isolated from formalin-fixed, paraffin-embedded tissue (FFPE)
Targets
G12X, G13X, Q61X, A146X and K117N
Controls
Target Positive Control included in the kit
LoB (Plasma)
KRAS-3X: G12/13: 13 droplets | Q61: 22 droplets | A146: 3 droplets | K117N: 1 droplet
Turnaround time
Bio-Rad: 4h (96 tests)
dPCR platforms
Bio-Rad: QX200™ Droplet Digital™ PCR System
Storage T°C
From -26°C to -16°C
Reference
IDKRAS-3X(b)-50
Certification
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