The IDEGFR-SENSI kit is a ready-to-use multiplex assay for digital PCR detection of common activating mutations (L858R, L861Q and exon 19 deletions) in theEGFR (Epidermal Growth Factor Receptor) gene.
This optimized reaction system offers a multiplexed solution enabling simultaneous amplification in a single PCR reaction ofEGFR mutations in each sample, including deletions inexon 19 and two substitutions, L858R and L861Q, in exon 21.
This IDEGFR-SENSI kit can be used in combination with the IDEGFR-RESIST and IDEGFR-RARE products.
The kit can be used on circulating DNA samples (cf-DNA) extracted from plasma, or on genomic DNA samples (gDNA) isolated from formalin-fixed, paraffin-embedded (FFPE) tissue.
Sample
Circulating free DNA (cf-DNA) extracted from plasma or genomic DNA (gDNA) isolated from formalin-fixed, paraffin-embedded tissue (FFPE)
Targets
L861Q, L858R and Del19
Controls
Target Positive Control Included in the kit
LoB (Plasma)
BioRad: L861Q: 1 droplet | L858R-Del19: 1 droplet
Stilla: L861Q: 6 droplet crystals | L858R: 1 droplet crystal | Del19: 1 droplet crystal
Turnaround time
Bio-Rad: 4h (96 tests)
Stilla: 3h (12 tests)
dPCR platforms
Bio-Rad: QX200™ Droplet Digital™ PCR System
Stilla: 3-color naica® system with Sapphire chips
Storage T°C
From -26°C to -16°C
Reference
IDEGFR(b)SENSI-V3-50
IDEGFR(s)SENSI-50
Certification
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