The IDNRAS kit is a ready-to-use multiplex assay for the detection and quantification by digital PCR of mutations in codons 12 and 13 of exon 2 and codon 61 of exon 3 of the NRAS (NRAS proto-oncogene, GTPase) gene.
This optimized reaction system offers a multiplexed solution enabling simultaneous amplification of NRAS mutations at positions G12X, G13X and Q61X for each sample in a single PCR reaction .
The kit can be used on circulating DNA samples (cf-DNA) extracted from plasma, or on genomic DNA samples (gDNA) isolated from formalin-fixed, paraffin-embedded (FFPE) tissue.
Sample
Circulating free DNA (cf-DNA) extracted from plasma or genomic DNA (gDNA) isolated from formalin-fixed, paraffin-embedded tissue (FFPE)
Targets
Exon 2: G12X and G13X
Exon 3: Q61X
Controls
Target Positive Control included on the kit
LoB (Plasma)
Bio-Rad: G12/13 mut: 2 droplets | Q61 mut: 3 droplets
Stilla: G12/13 mut: 3 droplet crystals | Q61 mut: 2 droplet crystals
Turnaround time
Bio-Rad: 4h (96 tests)
Stilla: 3h (12 tests)
dPCR platforms
Bio-Rad: QX200™ Droplet Digital™ PCR System
Stilla: 3-color naica® system with Sapphire chips
Storage T°C
From -26°C to -16°C
Reference
IDNRAS(b)-50
IDNRAS(s)-50
Certification
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