The IDBRAF-CNS kit is a ready-to-use multiplex test for the detection by digital PCR of mutations present in exon 15 (V600E) of the BRAF (B-Raf proto-oncogene, serine/threonine kinase) gene and the duplication of its tyrosine kinase domain (Exon 9-18) accompanying the fusion between the KIAA1549-BRAF genes.
This optimized reaction system offers a multiplexed solution enabling simultaneous amplification of the BRAF V600E (c.1799T>A) mutation and the KIAA1549-BRAF gene fusion in a single PCR reaction for each sample.
The kit can be used on circulating DNA samples (cf-DNA) extracted from plasma or on genomic DNA samples (gDNA) isolated from formalin-fixed, paraffin-embedded (FFPE) tissue.
Sample
Circulating free DNA (cf-DNA) extracted from plasma or genomic DNA (gDNA) isolated from formalin-fixed, paraffin-embedded tissue (FFPE)
Targets
V600E mutation (c.1799>A) and KIAA1549-BRAF fusion
Controls
Target Positive Control included in the kit
LoB (Plasma)
Bio-Rad: V600E: 3 droplets
Stilla: V600E: 1 droplet crystal
Turnaround time
Bio-Rad: 4h (96 tests)
Stilla: 3h (12 tests)
dPCR platforms
Bio-Rad: QX200™ Droplet Digital™ PCR System
Stilla: 3-color naica® system with Sapphire chips
Storage T°C
From -26°C to -16°C
Reference
IDBRAF-CNS(b)-25
IDBRAF-CNS(s)-25
Certification
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