The IDEGFR-RARE kit is a ready-to-use multiplex test for digital PCR detection of activating or resistance mutations (G719X, Insertions exon 20 and S768I) in theEGFR (Epidermal Growth Factor Receptor) gene.
This optimized reaction system offers a multiplexed solution for the simultaneous amplification of EGFR mutations, including G719X, S768I and exon 20 insertions, in a single PCR reaction for each sample.
This IDEGFR-RARE kit can be used in combination with the IDEGFR-SENSI and IDEGFR-RESIST products.
The kit can be used on circulating DNA samples (cf-DNA) extracted from plasma or on genomic DNA samples (gDNA) isolated from formalin-fixed, paraffin-embedded (FFPE) tissue.
Sample
Circulating free DNA (cf-DNA) extracted from plasma or genomic DNA (gDNA) isolated from formalin-fixed, paraffin-embedded tissue (FFPE)
Targets
G719X in Ex18 & Ins20/S768I in Ex20
Controls
Target Positive Control included in the kit
LoB (Plasma)
Bio-Rad: G719X: 3 droplets | Exon 20: 2 droplets
Stilla: G719X: 3 droplet crystals | Ins20: 3 droplet crystals | S768I: 1 droplet crystal
Turnaround time
Bio-Rad: 4h (96 tests)
Stilla: 3h (12 tests)
dPCR platforms
Bio-Rad: QX200™ Droplet Digital™ PCR System
Stilla: 3-color naica® system with Sapphire chips
Storage T°C
From -26°C to -16°C
Reference
IDEGFR(b)RARE-50
IDEGFR(s)RARE-50
Certification
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