The IDFGFR1-CNS kit is a ready-to-use multiplex assay for the detection and quantification by digital PCR of recurrent mutations and tandem duplication of the tyrosine kinase domain (TKD) of the FGFR1 (fibroblast growth factor receptor 1) gene.
This optimized reaction system offers a multiplexed solution enabling simultaneous amplification in a single PCR reaction of the N546K and K656E mutations,exon 8 (non-duplicated reference) andexon 16 (duplication) of the FGFR1 gene for each sample.
The kit can be used on circulating DNA samples (cf-DNA) extracted from plasma or on genomic DNA samples (gDNA) isolated from formalin-fixed, paraffin-embedded (FFPE) tissue.
Sample
Circulating free DNA (cf-DNA) extracted from plasma or genomic DNA (gDNA) isolated from formalin-fixed, paraffin-embedded tissue (FFPE)
Targets
Exon 12: N546K
Exon 14: K656E
TKD duplication
Controls
Target Positive Control Included in the kit
LoB (Plasma)
Bio-Rad: N546K/K656E: 1 droplet
Stilla: N546K/K656E: 3 droplet crystals
Turnaround time
Bio-Rad: 4h (96 tests)
Stilla: 3h (12 tests)
dPCR platforms
Bio-Rad: QX200™ Droplet Digital™ PCR System
Stilla: 3-color naica® system with Sapphire chips
Storage T°C
From -26°C to -16°C
Reference
IDFGFR1-CNS(b)-25
IDFGFR1-CNS(s)-25
Certification
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